SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   Manifest File for Whole Genome Sequencing (http://seqanswers.com/forums/showthread.php?t=74786)

KyuzoSeq 03-13-2017 11:04 PM

Manifest File for Whole Genome Sequencing
 
Hi,

I've been looking for the PCR regions or 'manifest file' for a whole genome sequencing kit? In an example there hundreds of WGS in 1000G project. They provide also exomes with BED files of targets. But there is no such info for genomes. Is there any thing that I missed? Is there a kit for whole genome sequencing and so its manifest file; or WGS is entirely different process than WES?

As a summary where do I find manifest like file for illumina's WGS or ex. 1000G project's?

:confused:

tristan dubos 03-13-2017 11:55 PM

Hi ,

I think the "BED files" for WGS you are looking is the genome reference used to align the reads.
Best,

Tristan

finswimmer 03-14-2017 09:17 PM

Quote:

Originally Posted by KyuzoSeq (Post 205229)
As a summary where do I find manifest like file for illumina's WGS or ex. 1000G project's?

As you are interessted in the whole genome sequence you wouldn't find any manifest file for WGS. The manifestfile defines the region to be analyzed after you received your fastq files. So in WGS you say: "Compare to the complete reference genome".

fin swimmer


All times are GMT -8. The time now is 03:56 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.