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-   -   NEWBIE question: does the 5' adapter mean forward primer? (http://seqanswers.com/forums/showthread.php?t=34067)

arkilis 09-26-2013 05:41 PM

NEWBIE question: does the 5' adapter mean forward primer?
 
As in the title.

3' adapter == reverse primer
5' adapter == foward primer

Is that right? Thanks!:cool:

Kennels 09-26-2013 06:13 PM

No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

arkilis 09-26-2013 08:37 PM

Quote:

Originally Posted by Kennels (Post 117349)
No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

thx~~ it is much clear to me now


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