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-   -   Ion PI™ Controls usage with Ion Torrent PGM (http://seqanswers.com/forums/showthread.php?t=90083)

Alex Alexl 07-09-2019 05:44 AM

Ion PI™ Controls usage with Ion Torrent PGM
 
Hello,
Does anybody know about compatibility between control sphere from Ion PI™ Controls 200 Kit and PGM in terms of sphere size, sequencing primer and so on? I need a control sphere for PGM but it looks like there is just Ion PI control kit with premade libraries and spheres. Is there any other control sphere kit for PGM?

r.rosati 07-09-2019 01:04 PM

Hi,
you're right, the v3 version of the Ion PGM™ Controls Kit doesn't have any Control Ion Spheres. The v2 version did.
Well size is notably bigger in PGM chips compared to Proton's PI, so I would say that the ISPs are not interchangeable, unfortunately. You might end up losing them during the run, I suppose.
An aliquot of Control ISPs comes with PGM sequencing kits - although they are barely more than what's needed for the kit's normal usage.
Did you need to make a test run with only control ISPs, as opposed to the normal amount of control ISPs that go in a sequencing run? Thermo engineers do use full control runs to check the machines at installation, so perhaps Thermo might be able to help?

Alex Alexl 07-10-2019 12:53 AM

Thanks a lot. Yes I need to run full chip with control IPS.


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