SEQanswers (
-   Sample Prep / Library Generation (
-   -   help with TruSeq (

upendra_35 06-08-2011 10:14 AM

help with TruSeq
1 Attachment(s)
I have recently used TruSeq kit for making 8 of Brassica RNAseq libraries and after amplification (15 cycles) and running on the gel (1% Agarose) i found that 5 out of 8 libraries have a broader range of fragments (smear) compared to the rest of the 3 libraries. I don't know why and the only reason i can think of possible over-amplification. But if it is so why only 5 and not all 8 have this problem. Any help is appreciated.

I have attached the picture of the gel for easy understanding.


pmiguel 06-08-2011 10:27 AM

The picture you attach looks to my viewer (FoxIt pdf viewer) like it has been processed through some bizarre filter to make it look like a scanning electron micrograph. That is off-putting -- but also you don't give the marker sizes.


mnkyboy 06-08-2011 11:03 AM

You might want to try repurifying them with the beads.

upendra_35 06-08-2011 11:27 AM

1 Attachment(s)
Hi pmiguel, marker sizes added now.

All times are GMT -8. The time now is 01:58 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.