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-   -   NEBNext Ultra-Directional RNA Library Prep Kit (http://seqanswers.com/forums/showthread.php?t=47848)

anna_m 10-29-2014 01:18 AM

NEBNext Ultra-Directional RNA Library Prep Kit
 
Hello,
Has anyone tried the NEBNext Ultra Directional RNA Library Prep Kit? If so, how were your final libraries? Any feedback on this would be great, thanks.

https://www.neb.com/products/e7420-n...t-for-illumina

All the best,
Anna.

egalvez 01-28-2015 12:56 PM

Hi Anna a little bit late the reply,
I am using the protocol and it's really good when you have a good RNA amount, I used this kit for gut-microbiota in combination with Ribozero and I got consistent results, I have to say that I do not have experience with others, but in comparison with an in-home ligation protocol this NEB performs well …it’s fast and isn’t so expensive.

Best and good luck

aubrie 10-18-2016 12:01 PM

Does anyone have experience with automating this kit on the EpMotion?

Thank you,
Aubrie


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