Hi all,
I use SOLiD4 , its protocol is Multiplex(barcode) Frangment run.
Usually my sample is ChIP sample.
Though I amplificated 12 libraries and run as usual last time, 4 of 12 libraries were little assign. The number of 4 was ten times or more as little as that of others.
Of course I checked amout of P1-P2fragment by qPCR and collect libraries same range by e-gel.
After I did ePCR using same amout of libraries as a template, I got good results in WFA.
Does anybody know such a trouble?
I have no idea but there is a possibility that the quality of ChIP DNA was bad.
However I cehcked those libraries has P1 and P2 by qPCR and size range was collect.(All libraries were same range.)
Nevertheless, after sequencing the number of assign beads was much different. I couldn't understand.
Other possiblity is the sequence of barcode?? One barcode is easy to assign???
Sorry, but please help me...
I use SOLiD4 , its protocol is Multiplex(barcode) Frangment run.
Usually my sample is ChIP sample.
Though I amplificated 12 libraries and run as usual last time, 4 of 12 libraries were little assign. The number of 4 was ten times or more as little as that of others.
Of course I checked amout of P1-P2fragment by qPCR and collect libraries same range by e-gel.
After I did ePCR using same amout of libraries as a template, I got good results in WFA.
Does anybody know such a trouble?
I have no idea but there is a possibility that the quality of ChIP DNA was bad.
However I cehcked those libraries has P1 and P2 by qPCR and size range was collect.(All libraries were same range.)
Nevertheless, after sequencing the number of assign beads was much different. I couldn't understand.
Other possiblity is the sequence of barcode?? One barcode is easy to assign???
Sorry, but please help me...
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