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-   -   Statistical Analyses of illumina Miseq 250PE Data (http://seqanswers.com/forums/showthread.php?t=83294)

hery 07-05-2018 01:26 PM

Statistical Analyses of illumina Miseq 250PE Data
 
Hello everyone !:)
I am a french PhD student in biology.

I am working on the impact of physicochemicals parameters on the composition of bacterial communities in natural breeding sites of mosquitoes larvae.
I collected 100 samples of breeding sites water, I measured their parameters( pH, salinity...) and by illumina Miseq sequençing i determined the bacterial communities composition of my 100 samples .
With these sequencing data I made an unifrac unweighted analysis and it clearly appears 2 clusters in PcoA.
I am new in the field of metagenomics.
I don't know which multivariate statistical tests to use (RDA, CCA, PERMANOVA?) in order to determine which physicochemicals variables explains the 2 clusters.
I thank you in advance for your reply.
best regards
hery


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