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-   -   ChIP - shearing with the Covaris S2 (http://seqanswers.com/forums/showthread.php?t=16554)

ETHANol 01-01-2012 08:17 AM

ChIP - shearing with the Covaris S2
 
I've been doing some optimization work with ChIP and the Covaris S2. I figure some people would probably be interested. All in all it appears to be a great instrument. Fragmentation of the chromatin is really even without bias towards open chromatin which is important for ChIP-seq. My only complaint is that it only takes one sample at a time, so if you have a lot of samples to process it can take a good amount of time. But the reproducibility is worth the extra time.

Anyway, it's too much to post here but it's on my blog at:
http://ethanomics.wordpress.com/2011/11/30/177/
http://ethanomics.wordpress.com/2011/12/28/202/
http://ethanomics.wordpress.com/chip...aris-protocol/

Thanks to Hamid at Covaris for his help. Phenomenal customer support.

Liting 07-08-2012 07:38 PM

Why I can't open the Links?
Can you give me the new links?Thanks !

ETHANol 08-09-2012 11:47 PM

The links seem to be fine. Are you still having this problem? Send me a PM if you like.


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