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-   -   BluePippin vs PippinPrep (http://seqanswers.com/forums/showthread.php?t=85364)

cement_head 10-31-2018 03:06 AM

BluePippin vs PippinPrep
 
Hello,

Initially SAGE marketed the PippinPrep, and later the BluePippin. These were targeted at two different size selection schemes (90 - 1500 bp) and BP for large fragments. Recently, the BluePippin has been listed as now being able to do fragments starting from 100 bp upwards to 50 Kbp.

Here's my question: Does the BluePippin now do everything that the PippinPrep can do? Or are there resolution/recovery differences between the two, even though they are listed as being able to recovery virtually the same size fragments?

Insight from people who either have both, or have used both is most welcome.

Thanks

kmcarr 10-31-2018 10:08 AM

Quote:

Originally Posted by cement_head (Post 221087)
Hello,

Initially SAGE marketed the PippinPrep, and later the BluePippin. These were targeted at two different size selection schemes (90 - 1500 bp) and BP for large fragments. Recently, the BluePippin has been listed as now being able to do fragments starting from 100 bp upwards to 50 Kbp.

Here's my question: Does the BluePippin now do everything that the PippinPrep can do? Or are there resolution/recovery differences between the two, even though they are listed as being able to recovery virtually the same size fragments?

Insight from people who either have both, or have used both is most welcome.

Thanks

We purchased a BluePippin long ago and it has always had the ability to resolve both long and short fragments, dependent upon which gel cassette and program was run. What the BluePippin has that the regular PippinPrep doesn't is the ability to perform Pulse Field Electrophoresis for resolution of larger molecules.

cement_head 10-31-2018 07:13 PM

Yeah, okay - but is the recovery of smaller (< 1.5 Kbp) bands from the PippinPrep better?

nucacidhunter 11-01-2018 01:32 AM

There is not any practical noticeable difference, though I have not done any systematic comparison.

kmcarr 11-01-2018 04:20 AM

Quote:

Originally Posted by cement_head (Post 221106)
Yeah, okay - but is the recovery of smaller (< 1.5 Kbp) bands from the PippinPrep better?

I have only ever used the BluePippin so can't make a comparison. I can say that I regularly isolated 200bp fragments using the BluePippin and was more than satisfied with the results.


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