In cDNA generated from poly-A reverse transcription of RNA, is it possible that PCR will detect incompletely spliced mRNA? How does one differentiate between something that is a legit isoform and an incompletely spliced molecule?
On amplifying using primers binding to the 5'UTR and the 3'UTR, I am seeing a faint longer band in addition to a band at the size I expected based on the predicted mature mRNA.
On amplifying using primers binding to the 5'UTR and the 3'UTR, I am seeing a faint longer band in addition to a band at the size I expected based on the predicted mature mRNA.
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