Hi
I am quite new in genomics and bioinformatics. I am a vet trying to do some research on pathogenic bacteria.
I have a simple and maybe stupid question.
I have the reference genome of a bacteria, and I want to find location of sequences (e.g. PCR primer sequences or any gene sequences).
I tried IGV and some DNAStar tools, but or I don't get results (i think due to mismatch), or an alignment is made and the "non overlapping" sequences are removed so I can't "see" where it is in the ref genome nor the connection with annotations.
Is there a tool to just simply open a ref and then input a sequence what "finds" it's place?
thanks
Zoli
I am quite new in genomics and bioinformatics. I am a vet trying to do some research on pathogenic bacteria.
I have a simple and maybe stupid question.
I have the reference genome of a bacteria, and I want to find location of sequences (e.g. PCR primer sequences or any gene sequences).
I tried IGV and some DNAStar tools, but or I don't get results (i think due to mismatch), or an alignment is made and the "non overlapping" sequences are removed so I can't "see" where it is in the ref genome nor the connection with annotations.
Is there a tool to just simply open a ref and then input a sequence what "finds" it's place?
thanks
Zoli
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