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-   -   Next Generation Sequencing for Dummies (http://seqanswers.com/forums/showthread.php?t=74469)

avierstr 02-27-2017 02:32 AM

Next Generation Sequencing for Dummies
 
Hi all,

I finally found some time to update my presentation from 2012. A lot changed in the NGS world in those years. I originally posted the link in http://seqanswers.com/forums/showthread.php?t=19067 but since the title does not cover the complete presentation, I've made a new post.

155 slides about the mostly used systems today, about applications (mainly for biology research) and some things to take into consideration and think about. The presentation and file with explanation can be downloaded from my webpage:
http://users.ugent.be/~avierstr/nextgen/nextgen.html

GenoMax 02-27-2017 04:32 AM

A very comprehensive effort. Thank you Andy!

This may be a good reference to go back to as technologies die out over time (e.g. 454, SOLiD).

rnaseq2017 03-23-2017 10:45 AM

Thanks!
 
Excellent for beginers like me!

Brian Bushnell 03-23-2017 02:03 PM

Thanks! This is also very useful for non-beginners.

shchaoguo 03-23-2017 03:13 PM

Thank you and very informative.

avierstr 03-27-2017 02:48 AM

I have posted an updated version on March 27th 2017.

Updates about:
- Oxford Nanopore
- Some minor adjustments here and there

Added info about:
- SeqLL True Single Molecule Sequencing (Helicos technology)
- 2D transcriptomics

Available :
http://users.ugent.be/~avierstr/nextgen/nextgen.html

avierstr 11-07-2017 04:59 AM

I have posted an updated version on November 7th 2017.

Updates about:
- added a slide (nr. 110) how errors are introduced in sequences
- Some minor adjustments here and there

Available :
http://users.ugent.be/~avierstr/nextgen/nextgen.html


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