Hi Friends
I am using TruSeq whole RNA sequencing kit. I used 50 ng input of earlier purified mRNA and gave a 15 cycle. At the end, the libraries were dissolved in 30 microliter of RSB buffer. Out of 4 libraries, one of the library is 1/10 of others in terms of concentration of dsDNA. Should I be worried? I have not yet done any bioanalyzer analysis.
Biochembug
I am using TruSeq whole RNA sequencing kit. I used 50 ng input of earlier purified mRNA and gave a 15 cycle. At the end, the libraries were dissolved in 30 microliter of RSB buffer. Out of 4 libraries, one of the library is 1/10 of others in terms of concentration of dsDNA. Should I be worried? I have not yet done any bioanalyzer analysis.
Biochembug