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lior lobel 07-17-2013 09:14 AM

rRNA sizes on Agarose gel
1 Attachment(s)
Hi All,

I ran a Agarsoe gel with my RNA samples and got the attached pattern.
23S rRNA and 16S rRNA are supposed to be ~3000 bps and ~1500 bps, however in this run I see 1500 and 750 bands.

Can this be a gel problem? Has anyone encountered this?



cliffbeall 07-19-2013 06:21 AM

RNA will not run on a gel exactly according to its size because it is single stranded and the secondary structure can be variable. To get an accurate size you need to use a denaturing gel (formaldehyde, urea, methyl mercury) and compare it to RNA standards.

Since you see 2 pretty sharp bands, your RNA is probably fine.

Edit: There is also a bioanalyzer chip for RNA that would be an option if you have access to an instrument.

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