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-   -   Bubbles in Miseq Flowcell (http://seqanswers.com/forums/showthread.php?t=44932)

DRYTCYV 07-13-2014 09:37 AM

Bubbles in Miseq Flowcell
 
Hi everyone,

I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I don’t know if you check the images in every Run, but for you, what kind of strange things you find in the images and assume that its normal?

Thanks

https://www.dropbox.com/s/32pva4lnde0zim4/Run%23C.png
https://www.dropbox.com/s/gvwxxr9rsg8yagc/Run%23B.png
https://www.dropbox.com/sc/er5vt25tb...e8-7hMm_EWELqa

dfhdfh 07-14-2014 12:03 AM

I observed similar things as well. These runs more or less failed with bad clustering efficiency. Illumina support is on the case but hasn't solved anything, yet (and I don't know whether the bubbles are the cause).

DRYTCYV 07-15-2014 08:15 AM

Hi dfhdfh,

Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the fluidic system.

No more feedback? anyone?

snetmcom 07-18-2014 06:07 PM

Bubbles are not that uncommon. It really depends on the frequency. I'd be curious if you find any tips.


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