Dear all,
I am performing ChIPseq experiments, and I am looking for an alternative for phenol-chloroform-isoamylalcohol elution/precipitation after the IP. I have tried Qiagen columns, but the recovery is 1/10 of phenol:chloroform:isoamylalcohol (measured by Qubit) . As I am quite restricted in starting material I cannot use more . Does anyone of you have experience with AMPURE XP beads at this step? I am wondering whether they will preform well, as DNA concentration is rather low (should be ~0.05 ng/µl at this step), and size is rather low as well (50 to >1000 bp, but enrichment between 100 - 500 bp).
Reasons that I want to omit the phenol stuff is problems with automated subsequent library preparation caused by tiniest amounts of residual phenol, and that I am pregnant.
Thanks a lot for your help!
I am performing ChIPseq experiments, and I am looking for an alternative for phenol-chloroform-isoamylalcohol elution/precipitation after the IP. I have tried Qiagen columns, but the recovery is 1/10 of phenol:chloroform:isoamylalcohol (measured by Qubit) . As I am quite restricted in starting material I cannot use more . Does anyone of you have experience with AMPURE XP beads at this step? I am wondering whether they will preform well, as DNA concentration is rather low (should be ~0.05 ng/µl at this step), and size is rather low as well (50 to >1000 bp, but enrichment between 100 - 500 bp).
Reasons that I want to omit the phenol stuff is problems with automated subsequent library preparation caused by tiniest amounts of residual phenol, and that I am pregnant.
Thanks a lot for your help!
Comment