I am modifying code that automatically guesses the scoring method used in a fastq file. I am using the wikipedia entry for guidance (http://en.wikipedia.org/wiki/FASTQ_format).
But the example they show has some discrepancies. In the top part of the image illumina-1.5+ scores start at position 66. But at the bottom of the image it starts at they start at 67.
The bigger problem I am having is that some fastqs that I am looking at have quality scores of 'i' which is not consistent with any of the scoring methods listed on wikipedia.
Can anyone clear this up for me? Is there a better source than wikipedia for this information?
Thanks.
But the example they show has some discrepancies. In the top part of the image illumina-1.5+ scores start at position 66. But at the bottom of the image it starts at they start at 67.
The bigger problem I am having is that some fastqs that I am looking at have quality scores of 'i' which is not consistent with any of the scoring methods listed on wikipedia.
Can anyone clear this up for me? Is there a better source than wikipedia for this information?
Thanks.
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