Hi!
I will start using Illumina. My idea is to use a protocol ddRADSeq, digest with two enzymes my genome. I do not have a reference genome. What criteria is the best to choose the enzymes? Is there a way to know how many fragments will I get?
Any recommendations will be welcome.
I will start using Illumina. My idea is to use a protocol ddRADSeq, digest with two enzymes my genome. I do not have a reference genome. What criteria is the best to choose the enzymes? Is there a way to know how many fragments will I get?
Any recommendations will be welcome.