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-   -   tophat paired end read alignment (http://seqanswers.com/forums/showthread.php?t=17789)

idyll_ty 02-18-2012 10:58 PM

tophat paired end read alignment
 
Hi, I am doing paired end alignment using tophat. Since I'm new to this program, I'm a little confusing about the format of the alignment output: accepted_hits.bam. How can I determine which read end it shows for a mate pair? How can I know the inner distance between the mate pairs? If the 7th column is * , does it mean one end aligned but the other end not?

Besides, is it -r option still required for paired end read mapping using Tophat? Though I didn't use this option, I still get the alignment result...

Look forward to your suggestions, and thanks for your help in advance!


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