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bernieball 04-06-2012 11:25 AM

Bioruptor shearing of PCR product
We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes.

Has anyone worked with PCR products vs. genomic DNA in a similar manner?

We will be using a Bioruptor UCD-200.

Thanks for your help!

jchung 11-25-2013 07:37 AM


I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb.


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