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-   -   Bioruptor shearing of PCR product (http://seqanswers.com/forums/showthread.php?t=19019)

bernieball 04-06-2012 11:25 AM

Bioruptor shearing of PCR product
 
We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes.

Has anyone worked with PCR products vs. genomic DNA in a similar manner?

We will be using a Bioruptor UCD-200.

Thanks for your help!

jchung 11-25-2013 07:37 AM

Bernie,

I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb.

Thanks!


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