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-   -   kmer content warning in FastQC (http://seqanswers.com/forums/showthread.php?t=29042)

vallejov 04-05-2013 11:10 AM

kmer content warning in FastQC
 
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I would be so grateful if someone wouldn't mind taking a look at the kmer content graph from FastQC. This is RNA-seq data after trimming and filtering based on quality scores. My questions are: Does the y-axis represent frequency? If it does why are these kmers so frequently found? What could they be? Transposons? DNA? I should mention, that there are no overrepresented sequences detected by FastQC.

Thank you in advance for your time!!!


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