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LMM 04-16-2018 12:32 PM

Sanger Sequencing for Community Analysis
I have environmental cDNA left over from a tricky qPCR project. Is it possible to use Sanger Sequencing of the ITS 5.8S rDNA region to observe the community at even the order level?

gringer 04-17-2018 05:32 PM

Unlikely. Sanger sequencing doesn't really work with variants at a frequency below about 20%, and any INDEL variation in the community is going to mess up the sequencing quite a lot.

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