I'm having to switch sample prep methods after collecting cell lysates. I lysed my cells with Dynabeads Direct mRNA lysis buffer (contains lithium) and now need to make sure to keep miRNAs, which can't be done with Dynabeads Direct mRNA.
Qiagen RNeasy kits will produce total RNAs, but require the RLT buffer (contains guanidinium) for column binding, so I figure diluting my cell lysates with RLT buffer should do the trick. However, when I do this, I get a cloudy suspension, which disappears after addition of 70% ethanol.
Has anyone ever tried something like this? What was the yield and RNA quality afterwards?
Qiagen RNeasy kits will produce total RNAs, but require the RLT buffer (contains guanidinium) for column binding, so I figure diluting my cell lysates with RLT buffer should do the trick. However, when I do this, I get a cloudy suspension, which disappears after addition of 70% ethanol.
Has anyone ever tried something like this? What was the yield and RNA quality afterwards?
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