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We have only just started using the Bioanalyzer RNA 6000 Nano chips, and I haven't seen a good trace yet. Today's was very strange....
There is a main peak at ~40 seconds that blows the rest of the peaks away (one was over 1000 FU). The software is calling it 18S, but there isn't a corresponding 28S peak.
I've attached a PDF of the four traces.
I loaded 1 ul of total RNA, with concentrations of 7.2 to 31.6 ng/ul as measured by Qubit. These samples have been in -80 since May (2 months), made on 5/8/14 and thawed once on 5/9/14 to make TruSeq RNA libraries (which worked beautifully). Any thoughts on that huge peak? Thank you for your insight!
There is a main peak at ~40 seconds that blows the rest of the peaks away (one was over 1000 FU). The software is calling it 18S, but there isn't a corresponding 28S peak.
I've attached a PDF of the four traces.
I loaded 1 ul of total RNA, with concentrations of 7.2 to 31.6 ng/ul as measured by Qubit. These samples have been in -80 since May (2 months), made on 5/8/14 and thawed once on 5/9/14 to make TruSeq RNA libraries (which worked beautifully). Any thoughts on that huge peak? Thank you for your insight!
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