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-   -   Creating Phylogenetic tree from MAF format file (http://seqanswers.com/forums/showthread.php?t=22894)

mez 08-29-2012 07:40 AM

Creating Phylogenetic tree from MAF format file
 
I hope this is relevant to the discussions here.

I am working on a metagenome project and have genome sequences of several strains of a bacteria species, un-assembled, but in contig reads. I used the mugsy program to align them and I have been trying to find a tool to create a tree out of the MAF format file I generated, but I am having issues because of the nature of the original sequences being in contigs rather than assembled.

I am hoping someone jas worked with unassembled genomes with contig reads and could be able to point me to how I could generate an acceptable multi-alignment format from the data

Thanks as always.

mez.

themerlin 08-29-2012 12:37 PM

I'm a little unclear about your question. If you have contigs, then you are working with an assembly. Do you mean that you are working with overlapping paired-end reads?

mez 08-31-2012 01:12 PM

Sorry, I guess I should say the reads have been assembled into contigs, I was having trouble aligning the genomes of the different strains, but I am making progress on that now. Just trying to figure how best to use the MAF format file generated to create a phylogenetic tree.

themerlin 09-04-2012 10:02 AM

I'm not sure that you can create a tree from a MAF file with overlapping reads. With Mugsy, you usually provide separate files for each genome and then infer a tree from sequence conserved in each block. If you can get your MAF file in this format, you can create a FASTA alignment with Galaxy or with scripts in the bx-python package (https://bitbucket.org/james_taylor/bx-python/wiki/Home).


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