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Ghada 02-05-2016 12:33 AM

3500 genetic analyzer low quality value
3 Attachment(s)
Dear professor
we have a new 3500 genetic analyzer that sanger/dye terminator from applied biosystem. All amplicon have e low to medium quality value the same site 75-85 bp including the control PGEM, however the seq standard is ok. please check attached files
Any comments

MU Core 02-08-2016 07:25 AM

Ghada, you're observing the presence of "dye blobs" which is the result of poor removal of unincorporated dye terminators from the post-sequencing reaction. A common issue in dye terminator sequencing. You'll want to revisit your reaction clean-up method to see if changes can be made to optimize the method. Or, you may want to consider a different approach to reaction clean-up. Performing a google search with "removing dye blobs" will provide you with information on the topic.

Ghada 02-10-2016 09:40 PM

thank you MU core for your help.

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