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prepagam 03-27-2012 05:20 PM

Bioruptor - DNA in TE?
Various protocols for bioruptor shearing suggest that you reconstitute your DNA to your desired concentration in TE (e.g. 1ug DNA in 100ul TE). My DNA samples were eluted in Qiagen's AE elution buffer, and I wonder if I can shear in AE? I have little DNA for my samples (200-400ng), so I wasn't planning an ethanol purification step to concentrate them down, rather just use the eluted DNA in AE directly. Is this likely to be a problem?

I'm preparing DNA for library prep for Illumina paired end sequencing - using Biooscientific barcodes and library prep kit.

caesar1970 04-10-2012 09:17 PM

Yes, you can shear in AE buffer. It is very similar to TE buffer and should not be a problem at all.

NextGenSeq 04-11-2012 09:04 AM

It's TE without the E (EDTA)

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