Hi there,
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that topic, but couldn't find relevant infos regarding RNA-seq or ChIP-seq libraries.
I use 6 bp in-line (front-) barcodes on Illumina PE adapters and wonder if ligation/amplification biases could be affecting the results.
Thank you for the feedback in advance!
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that topic, but couldn't find relevant infos regarding RNA-seq or ChIP-seq libraries.
I use 6 bp in-line (front-) barcodes on Illumina PE adapters and wonder if ligation/amplification biases could be affecting the results.
Thank you for the feedback in advance!