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apeters6 03-27-2018 08:32 AM

Streptavidin Bead Quantitation
Hi Everyone!

I am trying to evaluate how well our new robot handles bead clean-ups with streptavidin beads. I would like to objectively quantitate bead carry over (after removal of supernatant) rather than just visually assessing. Any ideas?


nucacidhunter 03-27-2018 02:36 PM

Counting with a hemocytometer.

austinso 04-01-2018 08:12 PM

more context is needed, but you could:

1. use A600 as a measure of particulate carryover within the supernatant
2. pre-bind beads with biotin-fluorescein, then use biotin to displace to get carryover in terms of biotin-binding equivalents using a standard curve (fluorescence reader required)

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