I recently submitted the GecKO library (~120K guides) for a MiSeq Nano run to check diversity (and mostly to check my custom seq primer etc. was working). It performed 1.3M reads, so about 10x the library size. I realize this is not nearly enough, since it's recommended to do 100x the library size. Even with 1/10th of the recommended depth, I had about 2.5% unread guides (rec is <0.5%). Do you think I can go ahead with this library? I really don't want to spend more money to do a 12M+ run. Thoughts?
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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