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gLAH 04-16-2019 06:56 AM

UltraClean PCR purification kits
 
We are having continuing issues with background bacterial sequence contamination in our MiSeq sequencing data that we think we have tracked to the min-elute columns we use to purify and concentrate our dsDNA products prior to library prep. We wanted to try a Mo Bio UltraClean PCR clean-up product - Qiagen now has Mo Bio product but only supplies the PCR clean-up in a 96-well format and we need individual columns.
Does anyone know of an alternative source or substitution for these "clean" purification columns?
Thanks!
gLAH

luc 04-16-2019 06:19 PM

PCR cleanup should be comparatively "easy" and all the usual manufacturers should have good options.
Qiagen QIAquick PCR or Zymo DNA clean would be some we have used.

microgirl123 04-17-2019 11:42 AM

How about using bead-based cleanup and just skipping the columns entirely? I have heard of people having trouble with contamination of their PCR-grade water when they have samples with very low levels of template DNA.


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