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SongLi 01-22-2011 01:03 PM

Download from SRA archive
 
Hi,

I recently tried to download published Colorspace results from SRA archive. http://www.ncbi.nlm.nih.gov/sra

However, I could only download sequences in ATCG. About one month back, I downloaded some colorspace data, which are in 0123 format.

Where can I download reads in colorspace from SRA?

Thanks,
Song Li

simonandrews 01-23-2011 02:08 AM

You don't mention the specific sample you're interested in so it's difficult to give an exact answer. If the entry was deposited in base space then there won't be an option to download colorspace (since that wouldn't make any sense anyway).

If you have a .sra file instead of a direct fastq file then you can use the --dumpcs option to fastq-dump to get colorspace fastq files from the sra file.

SongLi 01-23-2011 06:29 AM

Hi Simon,

Thank you for the reply.

The story is a bit compilcated.

1. I downloaded some colorspace data about a month back. Then I accidentally deleted the file, so I wanted to download again. However, I can only get reads in sequence space from the same place where I downloaded before.

for example, when I was trying to download the modEncode data for Drosophila from this link, I only get sequence space data.

http://trace.ncbi.nlm.nih.gov/Traces...m=search&s=seq

2. SRA tools do not work for me for some reason, I have a post before on this forum, and SRA tool was not terminating for a single color space file for about 24 hrs. I found the above link, from which I can download the data (which I wanted to download again now) from SRA. But now, it's not working again.

Any suggestion?
Thanks
Song Li


Quote:

Originally Posted by simonandrews (Post 33422)
You don't mention the specific sample you're interested in so it's difficult to give an exact answer. If the entry was deposited in base space then there won't be an option to download colorspace (since that wouldn't make any sense anyway).

If you have a .sra file instead of a direct fastq file then you can use the --dumpcs option to fastq-dump to get colorspace fastq files from the sra file.


simonandrews 01-23-2011 11:45 PM

Quote:

Originally Posted by SongLi (Post 33427)
for example, when I was trying to download the modEncode data for Drosophila from this link, I only get sequence space data.

http://trace.ncbi.nlm.nih.gov/Traces...m=search&s=seq

There is indeed something odd about that entry. Even if you set the display preferences to show colorspace (and colorspace data shows up in the preview view), then you still seem to get base space when you download the fastq file. In this case it may be that the only way to get the colorspace data is from the sra file (which I agree is a pain).

It might be worth contacting NCBI since this could just be a bug in their download code.

mjpablo23 04-22-2011 09:55 AM

found the files?
 
Hi,
Were you able to download the colorspace files? I've been looking for them too.


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