SEQanswers (
-   Bioinformatics (
-   -   Tophat - Left Kept Reads (

jkozubek 07-26-2011 11:10 AM

Tophat - Left Kept Reads

I am wondering if Tophat generates a file of seq direct to the genome, prior to doing its mapping to the exon-exon junctions. I am hoping it does, such that i don't need to do a separate Bowtie run. Is that what the "left_kept_reads" file is, mapping reads that don't need exon-exon junctions to find the genome?


chenyao 07-27-2011 05:32 AM

I think in tophat_out files, "left_kept_reads" is mapped by bowtie, "left_kept_reads_seq1"(forget the name,the second one) is mapped by the rest reads which failed by bowtie.

jkozubek 07-27-2011 05:40 AM

Thanks for comments!

All times are GMT -8. The time now is 04:28 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.