Hi everybody,
During the library preparation it's possible to conserved Ampure beads during the different enzymatic reaction (it's the with beads strategy for improving yield, Fisher et al. Genome Biology 2011).
Nevertheless, after double size selection, the library is always eluted from Ampure before PCR amplification.
I want to know if it is possible to carry out a PCR directly on library not eluated from Ampure beads after size selection ?
During the library preparation it's possible to conserved Ampure beads during the different enzymatic reaction (it's the with beads strategy for improving yield, Fisher et al. Genome Biology 2011).
Nevertheless, after double size selection, the library is always eluted from Ampure before PCR amplification.
I want to know if it is possible to carry out a PCR directly on library not eluated from Ampure beads after size selection ?
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