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-   -   Oxford Nanopore @ ASHG (http://seqanswers.com/forums/showthread.php?t=24807)

Jeremy 03-06-2013 05:38 PM

Quote:

Originally Posted by Clive
([url
http://pathogenomics.bham.ac.uk/blog/2013/03/a-chat-with-oxford-nanopores-clive-brown-at-agbt-2013[/url])
“We didn’t even know that long reads were so important to people until after that AGBT presentation.”

Really? I find that hard to believe.

ECO 03-07-2013 10:42 AM

Waaaaaa we made promises we couldn't keep to get attention and funding...and now people expect us to keep them!

<tiny violin playing>

Seriously though, having been through the bring up of a single molecule sequencer...if the CTO is opining in a bar to a blogger about improving consensus accuracy by throwing in multiple types of pores...they are a LONG way from finalizing a chemistry, let alone stabilizing and commercializing it.

Happy to eat words. Actually I love having my pessimism proven wrong.

And I hope I'm not off Clive's list. I could do some long range clinical haplotyping pretty easily! ;)

GenoMax 03-07-2013 10:49 AM

Quote:

Originally Posted by ECO (Post 98510)
And I hope I'm not off Clive's list.

Perhaps not before this post.

But now you can't be sure :D

nickloman 03-07-2013 11:16 AM

Quote:

Originally Posted by ECO (Post 98510)
Seriously though, having been through the bring up of a single molecule sequencer...if the CTO is opining in a bar to a blogger about improving consensus accuracy by throwing in multiple types of pores...they are a LONG way from finalizing a chemistry, let alone stabilizing and commercializing it.

Just to note I added a clarification from Clive at the bottom of the post on that particular point...

Given that they are continuously screening new and better pores and that is in fact one of the cool things about a biological nanopore (just like other sequencing companies regularly screen for new polymerases), I didn't necessarily see that as reflective of where they are in the product development pipeline.

GenoMax 03-07-2013 11:31 AM

Quote:

Originally Posted by nickloman (Post 98517)
Given that they are continuously screening new and better pores and that is in fact one of the cool things about a biological nanopore (just like other sequencing companies regularly screen for new polymerases), I didn't necessarily see that as reflective of where they are in the product development pipeline.

No argument there.

We all hope they will stop the screening at some point and release the first product we can use.

GW_OK 10-18-2013 10:25 AM

So.
ASHG next week.
Any care to give odds on ONT:
A) Being present?
B) Announcing anything? (Announcing no announcements does not count)

To aid in your handicapping, a certain Clive Brown tweeted:
Quote:

Clive G. Brown ‏@Clive_G_Brown 9 Oct

I've reluctantly rejoined twitter purely so that I can make one tweet - when the appropriate time arises ...
Also, it appears the Minion is now sporting a new look.

GenoMax 10-18-2013 10:31 AM

Hopefully they are not going to wait till illumina's "right to negotiate" expire at end of 2016?

bstamps 10-18-2013 11:37 AM

I'm guessing


A) 100%
B) 75/25. Either they release something, or risk losing funding from investors at this point. My bet is that they release the Minion sooner than the Gridion.

GenoMax 10-18-2013 11:44 AM

Quote:

Originally Posted by bstamps (Post 119265)
I'm guessing


Either they release something, or risk losing funding from investors at this point. My bet is that they release the Minion sooner than the Gridion.

They just raised $64 Million this month: http://nextgenseek.com/2013/10/oxfor...es-64-million/ Clearly investors must have seen something that we do not have access to (yet).

BBoy 10-19-2013 12:50 AM

Quote:

Originally Posted by GenoMax (Post 119267)
They just raised $64 Million this month: http://nextgenseek.com/2013/10/oxfor...es-64-million/ Clearly investors must have seen something that we do not have access to (yet).

Maybe they were shown the foils from AGBT 2011 :-)

NextGenSeq 10-21-2013 11:58 AM

You could check their website.

https://www.nanoporetech.com/news/events
ASHG 2013. Oxford Nanopore will be exhibiting at ASHG 2013 in Boston, 22-26 October. http://www.ashg.org/2013meeting/

BBoy 10-21-2013 11:27 PM

Quote:

Originally Posted by NextGenSeq (Post 119475)
You could check their website.

https://www.nanoporetech.com/news/events
ASHG 2013. Oxford Nanopore will be exhibiting at ASHG 2013 in Boston, 22-26 October. http://www.ashg.org/2013meeting/

Hopefully more than mockups with plexiglass cutouts like last time around? Unless they show something this will start turning into the mother of all vaporware. Given that there are no leaks (and there were leaks before AGBT 2012) I am not too hopeful. Will be glad to eat my words.

gringer 10-22-2013 06:33 AM

Apparently position 654 in the exhibition centre at ASHG 2013:

http://www.ashg.org/cgi-bin/2013/ashg13fp.pl

[top left]

GW_OK 10-22-2013 10:10 AM

ONT put up some new movies in the past 4 days. New looks at the GridION cartridge as well as miRNA sequencing and protein detection.

DaleYuzuki 10-22-2013 02:25 PM

Oxford Nanopore at ASHG
 
1 Attachment(s)
Here at the ASHG tradeshow floor setup, was able to spot the Oxford booth. (Attached is a photo, and you can spot the stack of GridIONs on top of the table there.)

I heard a rumor yesterday about them but won't engage in any rumor-mongering. :)

Fred 10-23-2013 05:08 AM

Hi,

Why not try it ?

https://www.nanoporetech.com/technol...cess-programme

Fred

GW_OK 10-23-2013 07:41 AM

ONT is the Kool-aid Man busting through the wall:
"OH YEAH!"

PacBio shares already down 15%.
Some extra info at the bottom of the GenomeWeb article

The MinIons also appear to be re-usable! So, so cool.

GenoMax 10-23-2013 07:56 AM

Quote:

Originally Posted by GW_OK (Post 119711)
The MinIons also appear to be re-usable! So, so cool.

You have to send the MinIons in for recycle/reuse, if I remember correctly.

The article you linked says "participants can order additional flow cells for $999 a piece". I hope that is just during the trial and that the final cost will not be that high.

It would be difficult to keep tens on hand if they are going to cost $1K each.

rahulvrane 10-23-2013 07:56 AM

Maybe I am a bit new to the whole ONT discussion.. But from my read of the website, I see they talk about a 'Run till you get sufficient data' workflow. (Kinda enticing :D )

But has there been any mention of just how long each of these can be run and what the final pore density would be per chip for GridION and MinION?

Obviously the GridION would be a legit superior, but just how far behind is the MinION in performance (or throughput)? ... Any clues I might have missed so far or new ones from the latest ASHG?

Cheers!

GW_OK 10-23-2013 08:20 AM

Quote:

Originally Posted by GenoMax (Post 119712)
You have to send the MinIons in for recycle/reuse, if I remember correctly.

At the beginning of the article they mention the "flow cells" and "usb device" as being separate entities. Also, on the ONT website, they say you can dry down the flowcells before you send them back, a condition of the early access program. This, to me, seems as though the MinIon can accept single use cartridges.


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