Hi guys,
I don't understand why Illumina recommends an insert size of 200-800bp for Truseq ChIP library preparation when the protocol selects only 250-300 bp fragments for sequencing. Since Truseq index adaptor is about 63 bp long, only DNA fragments around 124-174 bp are sequenced. In that case, shouldn't we sonicate the DNA fragments around 150bp?
Cheers,
Melissa
I don't understand why Illumina recommends an insert size of 200-800bp for Truseq ChIP library preparation when the protocol selects only 250-300 bp fragments for sequencing. Since Truseq index adaptor is about 63 bp long, only DNA fragments around 124-174 bp are sequenced. In that case, shouldn't we sonicate the DNA fragments around 150bp?
Cheers,
Melissa
Comment