Hi folks,
We are new to genome sequencing, but we want to sequence fungal genomes using Hiseq. We were wondering if it is possible to include both mate-pair (2 or 3 kb) and paired-end (either 300 or 550 bp) libraries in one lane?
And if not possible to do so, which type of libraries (paired-end/mate-pair) shall we go with? Our fungi have their genome size of ca. 60 MB. At the moment, we are accessible to 2x150 but it can be changed to 2x250.
Thank you in advance for all advice!
We are new to genome sequencing, but we want to sequence fungal genomes using Hiseq. We were wondering if it is possible to include both mate-pair (2 or 3 kb) and paired-end (either 300 or 550 bp) libraries in one lane?
And if not possible to do so, which type of libraries (paired-end/mate-pair) shall we go with? Our fungi have their genome size of ca. 60 MB. At the moment, we are accessible to 2x150 but it can be changed to 2x250.
Thank you in advance for all advice!
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