Hi everyone,
Thanks for reading my post.
I know DESeq2 uses normalized mean count as independent filtering to improve the performance of multiple testing error correction. I would like to know is it possible I can add the criteria, |log2FC| > 1, together with normalized mean count as the independent filtering? If so, how could I add this criteria? by using "filter=" or "filterFun=" argument? How to write the command?
What I know now is to change the "lfcThreshold=" to 1, but this only change the null hypothesis from 0 to 1, not actually filter any genes.
Many Thanks in advance!
Thanks for reading my post.
I know DESeq2 uses normalized mean count as independent filtering to improve the performance of multiple testing error correction. I would like to know is it possible I can add the criteria, |log2FC| > 1, together with normalized mean count as the independent filtering? If so, how could I add this criteria? by using "filter=" or "filterFun=" argument? How to write the command?
What I know now is to change the "lfcThreshold=" to 1, but this only change the null hypothesis from 0 to 1, not actually filter any genes.
Many Thanks in advance!
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