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DavyK 08-29-2013 03:14 AM

Ion torrent PCR duplicates
Hi All,

I am looking at some targeted ion torrent sequencing data. Over 90% of mapped reads are PCR duplicates. Is this normal? I thought it might be because the target is very small (11kb, 16 individuals), but I honestly am not sure.

Any ideas, given the target size how many duplicates I should be expecting?


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