SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Genomic Resequencing (http://seqanswers.com/forums/forumdisplay.php?f=28)
-   -   SNP calling on samples sequenced with different technology (http://seqanswers.com/forums/showthread.php?t=84532)

weehzer 09-10-2018 04:49 AM

SNP calling on samples sequenced with different technology
 
Hi,
is it possible to compare SNPs from alignments of different samples, whereas the samples weren't sequenced on the same illumina platform?
Let's say some were sequenced with HiSeq 2x100, some with MySeq 2x300 and some others with NextSeq 2x150.
We were discussing this issue recently, as we found a paper saying that all of these technologies have (slightly) different sorts of sequencing errors and rates.

However, I also read papers were they used sequenced samples together with simulated reads from a reference genome for analysis. So I guess it should be fine.

Any comment and thoughts on the issue are highly appreciated.

Thanks a lot & kind regards!


All times are GMT -8. The time now is 08:44 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.