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rvgill 10-26-2016 05:33 AM

Making local Database

I am making my own database out of fasta files and need help on how to re-format my command line when creating these databases.

makeblastdb -in test.fasta -dbtype nucl -parse_seqids -out testDB

The file type outputs that I receive currently when I run it is :

What I need is a .nal file to run blast from the error I am receiving. Now do I get a .nal file when I combine multiple databases? I have run blast on a few files and have a couple of folders with all of them organized into it.
How do I combine databases to get one large one with a _____.nal file type?

GenoMax 10-26-2016 06:38 AM

Use the blastdb_aliastool. Help available at this page.

maubp 10-26-2016 07:05 AM

This is (almost) a duplicate of your other thread, where you have been given some help:

GenoMax 10-26-2016 07:08 AM

Sounds like here the question is about combining two (or more) separate databases into one alias (if that is what @rvgill meant to ask).

maubp 10-26-2016 07:26 AM

In which case its a near duplicate of @rvgill's third thread

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