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Are the adapters the same or will this make a difference. Thanks
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Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem. -
What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?Originally posted by hoisinjl View Post
It looks to me like the adapter sequences are different, and that it wouldn't work the other way around.
Any thoughts?Comment
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No, libraries prepared with single read kits are not compatible with paired end cluster generation kits and flowcells.Originally posted by gro-seeker View PostComment
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All right, thanks so much! We'll have to go with single reads then.Comment
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