Dear all,
We just performed an RNA-seq experiment from human samples. To get some idea of the quality, I ran Picard's CollectRNASeqMetrics.
In the output, I find 'MEAN_CV_COVERAGE'. The explanation for this value is also on the website: the median CV of coverage of the 1000 most highly expressed transcripts.
I'm not really sure what CV means. And how can I interpret this value? The value I get is 0.48. If I think of this as 48x coverage, this seems really a lot to me. Especially since visualisation with IGV shows me a lower coverage.
Any help will be appreciated!
Thanks
Lien
We just performed an RNA-seq experiment from human samples. To get some idea of the quality, I ran Picard's CollectRNASeqMetrics.
In the output, I find 'MEAN_CV_COVERAGE'. The explanation for this value is also on the website: the median CV of coverage of the 1000 most highly expressed transcripts.
I'm not really sure what CV means. And how can I interpret this value? The value I get is 0.48. If I think of this as 48x coverage, this seems really a lot to me. Especially since visualisation with IGV shows me a lower coverage.
Any help will be appreciated!
Thanks
Lien
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