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-   -   MiSeq run failed; low clustering, but good QC (http://seqanswers.com/forums/showthread.php?t=93933)

cement_head 04-18-2020 07:31 AM

MiSeq run failed; low clustering, but good QC
 
Have a strange situation, our latest MiSeq run failed for 16S - from a protocol (EMP @ ArgonneNatLabs) that we use all the time.

The clustering was unusually low (180 vs the expected 800) and we did a KAPA just prior to dilutions (those are correct, I checked). The run QC, Clusters %PF and all the obvious look very good - when the run finished, it did not demultiplex and says "failed".

I've contacted Illumina, but this being a Saturday, I thought I'd see if anyone has seen this before?

cement_head 04-23-2020 08:32 AM

Bad NaOH...

Made fresh & reloaded - now working.


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