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-   -   Multiplexing with ion torrent? (http://seqanswers.com/forums/showthread.php?t=15819)

singaporeseq 11-28-2011 01:00 AM

Multiplexing with ion torrent?
 
I am looking for a protocol or personal experience in doing multiplexing with ion torrent. Has anybody done this?
The app note is not very informative

krobison 11-28-2011 07:16 AM

What sort of samples are you trying to multiplex? It makes a big difference.

For amplicons, one route is to just design appropriate tags (ones designed for the 454 will work) into your primers.

For fragment libraries, NuGen has a kit and I believe Ion has their own similar kits.

singaporeseq 11-28-2011 07:38 AM

Thanks for the info. I was interested in both amplicons and also bacterial whole genome.
I wanted to see if anyone has experience with the ion barcode kits. I can't find much information on them.

ionman 11-30-2011 08:49 PM

Have you seen the Ion Express gDNA and Amplicon Library preparation guide posted on the Ion Community?
http://lifetech-it.hosted.jivesoftwa.../docs/DOC-1997


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