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-   -   Concentrating method for TruSeq lib prep in microRNA sequencing (http://seqanswers.com/forums/showthread.php?t=65688)

candicechu 01-20-2016 12:17 PM

Concentrating method for TruSeq lib prep in microRNA sequencing
 
Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples.

I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method?

Any suggestions are appreciated. Thanks!

nucacidhunter 01-20-2016 04:40 PM

SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.


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