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Kultamuna2 03-11-2018 06:53 AM

Flow cell
Hi! Is the flow cell analyzed in one go per base (as a whole), or scanned one part of the flow cell at a time, until all bases have been identified? How many cameras are there in llumina's instruments to image the flow cell? Thanks in advance!

JoeKutch 03-12-2018 06:41 AM

As far as I understand, the MiSeq, HiSeq 2500, and HiSeq 4000 each have a single camera. The flow cell is divided into lanes, swaths (left/right/center depending on the machine), surfaces (top and bottom), and tiles. A single tile is imaged at a time, except during focusing. The camera moves up a swath, imaging each tile's top surface. It does all the swaths for the top surface, then goes back and does the bottom surface for each tile.

The NextSeq is slightly different, with six cameras/sensors working as a single module, simultaneously imaging six different tiles.

I can't speak to the NovaSeq or iSeq.

Kultamuna2 03-12-2018 04:26 PM

Thanks a lot for the good answer, it helped a lot!

GW_OK 03-13-2018 06:11 AM

I do know Hiseq 2500's have two cameras. Here's a picture of the optical deck in case anyone is interested. You can see the green and red lasers at the left and top and the two cameras at angles on the right.

Click for bigger version.

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