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-   -   How to interpret this HS DNA library profile (http://seqanswers.com/forums/showthread.php?t=66677)

sajoshi 03-04-2016 11:04 AM

How to interpret this HS DNA library profile
 
1 Attachment(s)
Please see attached PDF and give your feedback. Are these good quality libraries for RNA seq.

Thanks,

nucacidhunter 03-04-2016 01:42 PM

They do not seem to be good libraries for sequencing. Fragment size distributions are different among libraries indicating issues with library prep which will affect downstream sequencing and analysis negatively. Four of the libraries have high level of prime/adapter-dimers. Libraries are also overloaded for HS Chip.


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