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-   -   FuPa reagent? (http://seqanswers.com/forums/showthread.php?t=81786)

jlove 04-12-2018 01:59 PM

FuPa reagent?
 
Can anyone explain how the FuPa reagent works? In the context of Illumina library preparation, The FuPa reagent is a proprietary reagent that is used to partially digest the primers and phosphorylate the amplicons for adapter ligation. Illumina told me it was proprietary.

Thanks in advance!

torben 04-16-2018 01:05 AM

Since it's proprietary no one outside Thermo really knows, but some good guesses can be found in this thread http://seqanswers.com/forums/showthread.php?t=27039

r.rosati 04-16-2018 08:30 AM

This patent record is a nice read regarding the FuPa reagent:
https://patents.google.com/patent/US8728728B2/en


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