SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   PhiX control for a custom made library (http://seqanswers.com/forums/showthread.php?t=74910)

ymym 03-19-2017 10:16 PM

PhiX control for a custom made library
 
I am preparing a custom made PCR-based amplicon library.
I will use custom primers for the read in miseq but since it is a low diversity library, I need some control as PhiX. However, PhiX works only with illuminas primers and will not work with my custom made primers.
What can I use instead of PhiX?
Do you there is an option to add my custom primers to Illumina's read1 and read 2 standard primers and by this keep using the PhiX control?

thank you

GenoMax 03-20-2017 03:05 AM

That should be no problem. See this thread for more: http://seqanswers.com/forums/showthread.php?t=39717


All times are GMT -8. The time now is 08:16 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.